Asian Journal of Research in Chemistry

Jyoti Rai ¹, Sunil Singh ¹, Inamullah ¹, Nisha Choudhary ¹, Surabhi Sharma ¹, Hemendra Gautam ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Invertis Institute of Pharmacy, Invertis University, Bareilly (U.P.)

Abstract

A new stability indicating absorption ratio method was developed and validated for the determination of Esomeprazole magnesium (ESO) and Domperidone (DOM) in capsule dosage form. The maximum wavelength (&#955; max) was found to be 299nm for ESO and 287nm for DOM. The iso-absorptive point of both the drug was found to be 290 nm. The linearity range was found to be 1-6 μg ml<SUP>-1</SUP>(r<SUP>2</SUP>= 0.998) and 5-30 &#181;g ml<SUP>-1</SUP>(r<SUP>2</SUP>= 0.999) for ESO and DOM respectively. The value of limit of detection and limit of quantification was 0.116&#181;gml<SUP>-1</SUP> and 0.386&#181;gml-1 for ESO and 0.657&#181;gml<SUP>-1</SUP> and 2.18&#181;gml<SUP>-1</SUP> for DOM respectively. Forced degradations were carried out under acid, base, thermal, photolytic and oxidative stress conditions. The method was satisfactorily validated as per ICH guideline. The result of study showed that the proposed spectrophotometric method is useful for the routine determination of ESO and DOM in its combined pharmaceutical dosage form.

Keywords

Esomeprazole Magnesium, Domperidone, Spectrophotometric Method, Forced Degradation Studies, Absorption Ratio Method

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Nisha Choudhary ¹, Inamullah ¹, Sunil Singh ¹, Jyoti Rai ¹, Surabhi Sharma ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Invertis Institute of Pharmacy, Invertis University. Bareilly (U.P.)

Abstract

An accurate, specific and precise new Absorption Ratio method was developed for the simultaneous determination of Lansoprazole and Naproxen. The method involved Q-absorption analysis based on the measurement of absorbance at two wavelengths, i. e max of Lansoprazole (284nm) and for Naproxen was (271nm) and Iso-absorptive point of both drugs (275nm). The linearity was found to be 10-35&#181;g/ml (r2 =0·999) for NAP and 5-30&#181;g/ml (r 2 =0·998) for LAN. Validation was performed as ICH guidelines for Linearity, accuracy, precision, LOD and LOQ. The limit of quantification of NAP was found to be 0·15&#181;g/ml and for LAN was 1·7&#181;g/ml. The limit of detection of NAP was found to be 0·04&#181;g/ml and for LAN was 0·5&#181;g/ml. The indicating proposed spectrophotometric method was accurate and precise for estimation of NAP and LAN in tablet dosage form

Keywords

Lansoprazole, Naproxen, Iso-absorptive Point

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Sunil Singh ¹, Nitin Dubey ¹, D. K. Jain ¹

Affiliations
1 College of Pharmacy, IPS Academy, Rajendar Nagar, Indore [Madhya Pradesh], IN

Abstract

Atorvastatin [R-(R*,R*)]-2-(4-fluorophenyl)-β, δ-dihydroxy-5-(1-methylethyl)-3-phenyl-4-[(phenyl amino)carbonyl]-1H-pyrrole 1-heptanoic acid an Anti-hyperlipidemia and Clopidogrel (2-Chloro phenyl, 6-7 dihydro4H thieno [3,2-c]pyridine-5-yl)acetic acid is an Anti-platelet drug and Aspirin (2-Acetoxy benzoic acid) an NSAIDs. This paper presents two methods i.e. first derivative spectrophotometery and multicomponent spectrophotometery for simultaneous estimation of these three drugs combination in pharmaceutical formulations. The first derivative amplitudes at 276, 226 and 222 nm were utilized for simultaneous estimations. The multicomponent amplitudes at 247, 220 and 235 nm were utilizatied. Proper selection of wavelength for estimations of one drug by other in derivative spectra and multicomponent lead to successful development of methods for simultaneous estimation. The results of analysis were validated statistically that included parameters such as precision, LOD, LOQ, recovery and robustness. Both methods were simple, economical, accurate, reproducible and precise.

Keywords

Simultaneous Determination, Derivative Spectroscopy, Multicomponent Method.

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Sunil Singh ¹, Jay Ram Patel ¹, Sarita Kare ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Oriental College of Pharmacy, Bhopal, M.P., IN

Abstract

A simple, accurate, sensitive and precise Ultraviolet spectrophotometric method has been developed for the determination of Piroxicam in tablet dosage form. The solutions of standard and sample were prepared in methanol. In the UV specrophotometric method, the quantitative determination of the drug was carried at 335 nm and the linearity range was found to be 2-12 μg/ml. The calibration graphs constructed at their wavelength of determination were found to be linear for specrophotometric methods. The proposed methods have been extensively validated statistically that included parameters such as linearity, accuracy, precision, LOD, LOQ, recovery and robustness. There was no significant difference between the performance of the proposed method regarding the mean values and standard deviations. The described methods can be readily utilized for analysis of pharmaceutical formulation.

Keywords

Method Development, Validation, Derivative Spectroscopy, Piroxicam.

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